2024

• Complex and non-sequential current signatures of a β-hairpin peptide confined in a nanopore
  Misa Yamaji, Mauro Chinappi, Blasco Morozzo della Rocca, Kenji Usui, Ryuji Kawano
  Analytical Chemistry, accepted.
  
  
• Microfluidic generation of cell-sized liposomes using a budding strategy
  Jiajue Ji, Ryuji Kawano
  Preprint in ChemRxiv 2024, DOI: 10.26434/chemrxiv-2024-4gnff-v2 [Link]
  
  
• De novo design of nanopores for single-molecule detection that incorporates α-helix peptides
  Masataka Usami, Zugui Peng, Yusuke Sekiya, Batsaikhan Mijiddorj, Izuru Kawamura, Ryuji Kawano
  Preprint in ChemRxiv 2024, DOI: 10.26434/chemrxiv-2024-wzql1 [Link]
  
  
• Harnessing DNA computing and nanopore decoding for practical applications: from informatics to microRNA-targeting diagnostics
  Sotaro Takiguchi, Nanami Takeuchi, Vasily Shenshin, Guillaume Gines, Anthony J. Geno, Jeff Nivala, Yannick Rondelez, Ryuji Kawano
  Chem. Soc. Rev., Published online 2024. doi:10.1039/d3cs00396e [Link]
  
  
• Controlled Self-Assembly of Vesicles by Electrospray Deposition
  Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Kaori Kuribayashi-Shigetomi, Shoji Takeuchi
  Small Structures 2300543. DOI: 10.1002/sstr.202300543 [Link]

• Long-term Stable Liposome Modified by PEG-lipid in Natural Seawater
  Kayano Izumi, Jiajue Ji, Keiichiro Koiwai, Ryuji Kawano
  ACS Omega 2024, 9, 9, 10958-10966. DOI: 10.1021/acsomega.3c10346 [Link][pdf][Press]
  
  
• Lipid Vesicle-Based Molecular Robots
  Zugui Peng, Shoji Iwabuchi, Kayano Izumi, Sotaro Takiguchi, Misa Yamaji, Shoko Fujita, Harune Suzuki, Fumika Kambara, Genki Fukasawa, Aileen Cooney, Lorenzo Di Michele, Yuval Elani, Tomoaki Matsuura, Ryuji Kawano
  Lab on a Chip 2024, 24, 996-1029. DOI: 10.1039/D3LC00860F [Link][pdf][Lab on a Chip HOT Articles 2023]
  
  
• Real-time monitoring of the amyloid β1–42 monomer-to-oligomer channel transition using a lipid bilayer system
  Yuri Numaguchi?, Kaori Tsukakoshi?, Nanami Takeuchi, Yuki Suzuki, Kazunori Ikebukuro, Ryuji Kawano
  (?equal contribution)
  PNAS Nexus 2024, 3, 1,  pgad437, DOI: 10.1093/pnasnexus/pgad437 [Link][pdf][Press]
  
  

2023

• The potential of nanopore technologies toward empowering biophysical research: Brief history, basic principle and applications
  Hirohito Yamazaki, Zugui Peng, Ryuji Kawano, Kan Shoji
  Biophysics and Physicobiology 2023, e210003, DOI: 10.2142/biophysico.bppb-v21.0003 [Link]
  
  

• Bottom-up creation of cell-free molecular systems: Basic research toward social implementation
  Izuru Kawamura, Ryuji Kawano, Tomoaki Matsuura
  Biophysics and Physicobiology 2023, e200042, DOI: 10.2142/biophysico.bppb-v20.0042 [Link]
  
  
• Structural fexibility of apolipoprotein E?derived arginine?rich peptides improves their cell penetration capability
  Yuki Takechi?Haraya, Takashi Ohgita, Akiko Usui, Kazuchika Nishitsuji, Kenji Uchimura, Yasuhiro Abe, Ryuji Kawano, Monika I. Konaklieva, Mart Reimund, Alan T. Remaley, Yoji Sato, Ken?ichi Izutsu, Hiroyuki Saito
  Scientific Reports 2023, 13, 19396, DOI: 10.1038/s41598-023-46754-0 [Link]

• Enhancement of cell membrane permeability by using charged nanoparticles and a weak external electric field
  Hideya Nakamura, Takumi Okamura, Masaya Tajima, Ryuji Kawano, Misa Yamaji, Shuji Ohsaki, Satoru Watano
  Physical Chemistry Chemical Physics 2023, 25, 32356-32363. DOI: 10.1039/D3CP03281G [Link]

• Simultaneous Recognition of Over- and Under-expressed MicroRNAs using Nanopore Decoding
  Sotaro Takiguchi, Fumika Kambara, Mika Tani, Tsuyoshi Sugiura, Ryuji Kawano
  Analytical Chemistry 2023, 95, 39, 14675–14685, DOI: 10.1021/acs.analchem.3c02560 [Link][pdf][Press][Cover]

• Structure-activity relationship study of helix-stabilized antimicrobial peptides containing non-proteinogenic amino acids
  Takahito Ito, Wakana Hashimoto, Nobumichi Ohoka, Takashi Misawa, Takao Inoue, Ryuji Kawano, Yosuke Demizu
  ACS Biomaterials Science & Engineering 2023, 9, 8, 4654–4661, DOI: 10.1021/acsbiomaterials.3c00759 [Link]

• Nanopore Filter: A Method for Counting and Extracting Single DNA Molecules using a Biological Nanopore
  ?Asuka Tada, ?Nanami Takeuchi, Kan Shoji, Ryuji Kawano
  (?equal contribution)
  Analytical Chemistry 2023, 95, 26, 9805–9812, DOI: 10.1021/acs.analchem.3c00573 [Link][pdf][Cover][Press1][Press2]

• Liposome deformation induced by membrane-binding peptides
  Kayano Izumi, Chihiro Saito, Ryuji Kawano
  Micromachines 2023, 14(2), 373, DOI: 10.3390/mi14020373 [Link][pdf][Press]

• Cell-Free Expression of De Novo Designed Peptides That Form β-Barrel Nanopores
  Shoko Fujita, Izuru Kawamura, Ryuji Kawano
  ACS Nano 2023, 17, 4, 3358-3367. DOI: 10.1021/acsnano.2c07970 [Link][pdf][Press 1][Press 2][Press 3][Cover][ACS Insights]

2022

• Pattern Recognition of microRNA Expression in Body Fluids using Nanopore Decoding at Sub-femtomolar Concentrations
  Nanami Takeuchi, Moe Hiratani, Ryuji Kawano
  JACS Au 2022, 2, 8, 1829-1838, DOI: 10.1021/jacsau.2c00117 [Link][pdf][Press 1][Press 2][Cover][ACS Insights]
  (Preprint in ChemRxiv, 2021, DOI: 10.26434/chemrxiv-2021-p71pl-v2 [Link])
  
  
• Effect of hydrophobic moment on membrane interaction and cell penetration of apolipoprotein E-derived arginine-rich amphipathic α-helical peptides
  Yuki Takechi-Haraya, Takashi Ohgita, Mana Kotani, Hiroki Kono, Chihiro Saito, Hiroko Tamagaki-Asahina, Kazuchika Nishitsuji,
  Kenji Uchimura, Takeshi Sato, Ryuji Kawano, Kumiko Sakai-Kato, Ken-ichi Izutsu, and Hiroyuki Saito
  Scientific Reports 2022, 12, 4959, DOI: 10.1038/s41598-022-08876-9 [Link]
  
  
• Single Polypeptide Detection using a Translocon EXP2 Nanopore
  ?Mitsuki Miyagi, ?Sotaro Takiguchi, Kazuaki Hakamada, Masafumi Yohda, Ryuji Kawano
  (?equal contribution)
  Proteomics 2022, 22, 5-6, 2100070, DOI: 10.1002/pmic.202100070 [Link][pdf][Press]
  (Contribution to a Special Issue on "Protein detection and characterization with nanopore sensors" )
  
  
• De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide
  Keisuke Shimizu, Batsaikhan Mijiddorj, Masataka Usami, Ikuro Mizoguchi, Shuhei Yoshida, Shiori Akayama, Yoshio Hamada,
  Akifumi Ohyama, Kenji Usui, Izuru Kawamura, Ryuji Kawano
  Nature Nanotechnology 2022, 17, 67-75,
  DOI: 10.1038/s41565-021-01008-w [Link][pdf][Behind the paper][Press 1][Press 2][Press 3]
  (Preprint in ChemRxiv 2020, DOI: 10.26434/chemrxiv.12286337.v1 [Link])

2021

• Simple Fabrication of Solid-State Nanopores on a Carbon Film
  Natsumi Takai, Kan Shoji, Tei Maki, Ryuji Kawano
  Micromachines 2021, 12, 1135, DOI: 10.3390/mi12091135 [Link][pdf]
  (Contribution to a Special Issue on "Frontiers in Micromachines in Japan")
  
  
• Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process
  Keiichiro Koiwai, Takashi Koyama, Soichiro Tsuda, Atsushi Toyoda, Kiyoshi Kikuchi, Hiroaki Suzuki, Ryuji Kawano
  eLife 2021, 10, e66954, DOI: 10.7554/eLife.66954 [Link]
  (Preprint in bioRxiv 2021, DOI: 10.1101/2021.01.10.426076 [Link])
  
  
• Nanopore Decoding for a Hamiltonian Path Problem
  Sotaro Takiguchi and Ryuji Kawano
  Nanoscale 2021, 13, 6192-6200, DOI: 10.1039/D0NR09031J [Link][pdf][Press]
  
  
• Lipid bilayer on a microdroplet integrated with a patterned Ag/AgCl microelectrode for voltage-clamp fluorometry of membrane transport
  Taishi Tonooka, Toshihisa Osaki, Koji Sato, Ryuji Kawano, Shoji Takeuchi
  Sensors and Actuators B: Chemical 2021, 334, 129643, DOI: 10.1016/j.snb.2021.129643 [Link]
  
  
• Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence
  Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu
  Molecules 2021, 26(2), 444, DOI: 10.3390/molecules26020444 [Link]
  
  

2020

• Rational Design of Helix-stabilized Antimicrobial Peptide Foldamers Containing α,α-Disubstituted Amino Acids or Side-Chain Stapling
  Motoharu Hirano, Chihiro Saito, Chihiro Goto, Hidetomo Yokoo, Ryuji Kawano, Takashi Misawa, and Yosuke Demizu
  ChemPlusChem 2020, 85, 12, 2731-2736, DOI: 10.1002/cplu.202000749 [Link]
  
  
• Analytical Model for Particle Capture in Nanopores Elucidates Competition among Electrophoresis, Electroosmosis, and Dielectrophoresis
  Mauro Chinappi, Misa Yamaji, Ryuji Kawano, and Fabio Cecconi
  ACS Nano 2020, 14, 11, 15816-15828, DOI: 10.1021/acsnano.0c06981 [Link]
  
  
• Microfiber-shaped programmable materials with stimuli-responsive hydrogel
  Nobuki Takeuchi, Shunsuke Nakajima, Koki Yoshida, Ryuji Kawano, Yutaka Hori, and Hiroaki Onoe
  Soft Robotics 2020, 0, 0, DOI: 10.1089/soro.2020.0038 [Link]
  
  
• Recent Advances in Liposome-Based Molecular Robots
  Kan Shoji and Ryuji Kawano
  Micromachines 2020, 11(9), 788, DOI: 10.3390/mi11090788 [Link]
  
  
• Analysis of membrane protein de-insertion-associated currents with nanoneedle-supported bilayers to discover pore formation mechanisms
  Kan Shoji, Ryuji Kawano, and Ryan J. White
  Langmuir 2020, 36, 34, 10012–10021, DOI: 10.1021/acs.langmuir.0c00833 [Link]
  
  
• Microfluidic formation of honeycomb-patterned droplets bounded by  interface bilayers via bimodal molecular adsorption
  Shougo Fujiwara, Kan Shoji, Chiho Watanabe, Ryuji Kawano, Miho Yanagisawa
  Micromachines 2020, 11(7), 701, DOI: 10.3390/mi11070701 [Link]
  
  
• Recessed Ag/AgCl Microelectrode-Supported Lipid Bilayer for Nanopore Sensing
  Kan Shoji, Ryuji Kawano, and Ryan J. White
  Analytical Chemistry 2020, 92, 15, 10856-10862, DOI: 10.1021/acs.analchem.0c02720 [Link]
  
  
• Recognition of Single-Point-Mutation using a Biological Nanopore
  Ping Liu and Ryuji Kawano
  Small Methods 2020, 2000101, DOI: 10.1002/smtd.202000101 [Link][pdf][Press 1][Press 2]
  
  
• Enhancement of direct membrane penetration of arginine-rich peptides by polyproline II helix structure
  Takashi Ohgita, Yuki Takechi-Haraya, Keisuke Okada, Saki Matsui, Misaki Takeuchi, Chihiro Saito, Kazuchika Nishitsuji, Kenji Uchimura, Ryuji Kawano, Koki Hasegawa, Kumiko Sakai-Kato, Kenichi Akaji, Ken-ichi Izutsu, Hiroyuki Saito
  BBA - Biomembranes 2020, 183403, DOI: 10.1016/j.bbamem.2020.183403 [Link]
  
  
• Competing Roles of Two Kinds of Ligand during Nonclassical Crystallization of Pillared-Layer Metal-Organic Frameworks Elucidated Using Microfluidic Systems
  Yoko Tanaka, Yu Kitamura, Ryuji Kawano, Kan Shoji, Moe Hiratani, Tetsuo Honma, Hikaru Takaya, Hirofumi Yoshikawa, Takaaki Tsuruoka, Daisuke Tanaka
  Chem. Eur. J. 2020, 26, 41, 8889-8896, DOI: 10.1002/chem.202002105 [Link]
  
  
• Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy
  Takafumi Ueno, Kento Niwase, Daisho Tsubokawa, Kosuke Kikuchi, Natsumi Takai, Tadaomi Furuta, Ryuji Kawano, Takayuki Uchihashi
  Nanoscale 2020, 12(15), 8166-8173, DOI: 10.1039/d0nr01121e [Link]
  
  
• Biological Nanopore Probe: Probing of Viscous Solutions in a Confined Nanospace
  Masaki Matsushita, Kan Shoji, Natsumi Takai, Ryuji Kawano
  J. Phys. Chem. B., 2020, 124, 12, 2410-2416, DOI：10.1021/acs.jpcb.9b11096 [Link][pdf]
  
  

2019

• Osmotic-Engine-Driven Liposomes in Microfluidic Channels
  Kan Shoji and Ryuji Kawano
  Lab on a Chip, 2019, 19, 3472-3480, DOI :10.1039/C9LC00788A [Link][pdf]

• DNA origami nanoplate-based emulsion with designed nanopore function
  Daisuke Ishikawa, Yuki Suzuki, Chikako Kurokawa, Masayuki Ohara, Misato Tsuchiya, Masamune Morita, Miho Yanagisawa,Masayuki Endo, Ryuji Kawano, Masahiro Takinoue
  Angew. Chem., Int. Ed., 2019, 58, 43, DOI : 10.1002/anie.201908392 [Link]
  
  

• Electrophysiological Analysis of Antimicrobial Peptides in Diverse Species
  Naoki Saigo, Kayano Izumi, and Ryuji Kawano
  ACS Omega, 2019, 4, 8, 13124-13130, DOI：10.1021/acsomega.9b01033 [Link][pdf]
  
  

• Electrophysiological Analysis of Membrane Disruption by Bombinin and its Isomer using Lipid Bilayer System
  Yusuke Sekiya, Keisuke Shimizu, Yuki Kitahashi, Akifumi Ohyama, Izuru Kawamura, and Ryuji Kawano
  ACS Appl. Bio. Mater., 2019, 2, 4, 1542-1548, DOI:10.1021/acsabm.8b00835 [Link][pdf][press]
  
  

• Spatially Resolved Chemical Detection with a Nanoneedle-Probe-Supported Biological Nanopore
  Kan Shoji, Ryuji Kawano, and Ryan J. White
  ACS Nano, 2019, 13(2), 2606-2614, DOI :10.1021/acsnano.8b09667 [Link][pdf]
  
  

2018

• Electrophysiological measurement of ion channels on plasma/organelle membranes using an on-chip lipid bilayer system
  Koki Kamiya, Toshihisa Osaki, Kenji Nakao, Ryuji Kawano, Satoshi Fujii, Nobuo Misawa, Masatoshi Hayakawa, and Shoji Takeuchi
  Scientific Reports, 2018, 17498, DOI:10.1038/s41598-018-35316-4 [Link]
  
  
• Nanopore Decoding of Oligonucleotides in DNA Computing
  Ryuji Kawano
  Biotechnology Journal, 2018, 1800091, DOI: 10.1002/biot.201800091 [Link][pdf]
  
  
• Spring-shaped stimuli-responsive hydrogel actuator with large deformation
  Koki Yoshida, Shunsuke Nakajima, Ryuji Kawano, Hiroaki Onoe
  Sensors and Actuators B-Chemical, 2018, 272, 361, DOI: 10.1016/j.snb.2018.05.174[Link]
  
  
• Microfluidic Formation of Double-stacked Lipid Bilayers by Controlling the Water-Oil Interface
  ?Kan Shoji and Ryuji Kawano
  Micromachines, 2018, 9(5), 253, DOI: 10.3390/mi9050253 [Link][pdf]
  
  
• Channel Current Analysis Estimates the Pore-formation and the Penetration of Transmembrane Peptides
  Yusuke Sekiya, Shungo Sakashita, Keisuke Shimizu, Kenji Usui and Ryuji Kawano
  Analyst, 2018, 143(15), 3540-3543, DOI: 10.1039/C8AN00243F [Link][pdf][Cover]
  
  
• DNA Logic Operation with Nanopore Decoding To Recognize MicroRNA Patterns in Small Cell Lung Cancer
  Moe Hiratani and Ryuji Kawano
  Analytical Chemistry, 2018, 90(14), 8531-8537, DOI: 10.1021/acs.analchem.8b01586 [Link][pdf][Press1][Press2][Virtual Issue]
  
  
• Design of protein-responsive micro-sized hydrogels for self-regulating microfluidic systems
  Mayu Hirayama, Kazuhiro Tsuruta, Akifumi Kawamura, Masayuki Ohara, Kan Shoji, Ryuji Kawano and Takashi Miyata
  Journal of Micromechanics and Microengineering, 2018, 28(3), DOI: 10.1088/1361-6439/aaa876[Link]
  
  
• Well-controlled cell-trapping systems for investigating heterogeneous cell-cell interactions
  Koki Kamiya, Yuta Abe, Kosuke Inoue, Toshihisa Osaki, Ryuji Kawano, Norihisa Miki and Shoji Takeuchi
  Advanced Healthcare Materials, 2018, 7(6), 2192-2659, DOI: 10.1002/adhm.201701208 [Link][Cover]
  
  
• Synthetic Ion Channels and DNA Logic Gates as Components of Molecular Robots (Invited Minireview)
  Ryuji Kawano
  ChemPhysChem, 2018, 19(4), 359-366, DOI: 10.1002/cphc.201700982 [Link][PDF][Cover]

2017

• Analysis of Pore Formation and Protein Translocation Using Large Biological Nanopores
  Hirokazu Watanabe, Alberto Gubbiotti, Mauro Chinappi, Natsumi Takai, Koji Tanaka, Kouhei Tsumoto and Ryuji Kawano
  Analytical Chemistry, 2017, 89(21), 11269–11277, DOI: 10.1021/acs.analchem.7b01550[Link] [pdf]
  
  
• MicroRNA Detection at Femtomolar Concentration by a Biological Nanopore with an Isothermal Amplification
  Haolin Zhang, Moe Hiratani, Kentaro Nagaoka and Ryuji Kawano
  Nanoscale, 2017, 9, 16124-16127, DOI: 10.1039/C7NR04215A [Link] [pdf][Press]
  
  
• pH Regulates Pore Formation of a Protease Activated Vip3Aa from Bacillus thuringiensis
  Thittaya Kunthic, Hirokazu Watanabe, Ryuji Kawano, Yoshikazu Tanaka, Boonhiang Promdonkoy, Min Yao and Panadda Boonserm
  Biochimica et Biophysica Acta (BBA) - Biomembranes, 2017, 1859, 2234-2241, DOI: 10.1016/j.bbamem.2017.08.018[Link]
  
  
• Serial DNA Relay in DNA Logic Gates by electrical Fusion and Mechanical Splitting of Droplets
  Hiroki Yasuga, Kosuke Inoue, Ryuji Kawano, Masahiro Takinoue, Toshihisa Osaki, Koki Kamiya, Norihisa Miki and Shoji Takeuchi
  PLOS ONE, 2017, 12(7), DOI: 10.1371/journal.pone.0180876 [Link]
  
  
• Nanopore Logic Operation with DNA to RNA Transcription in a Droplet System
  Masayuki Ohara, Masahiro Takinoue and Ryuji Kawano
  ACS Synthetic Biology, 2017, 6(7), 1427-1432, DOI: 10.1021/acssynbio.7b00101 [Link] [pdf][Press]
  
  
• Stimuli-responsive Hydrogel Microfibers with Controlled Anisotropic Shrinkage and Cross-sectional Geometries
  Shunsuke Nakajima, Ryuji Kawano and Hiroaki Onoe
  Soft Matter, 2017, 13, 3710-3719, DOI: 10.1039/C7SM00279C [Link]
  
  
• Metal-Organic Cuboctahedra for Synthetic Ion Channels with Multiple Conductance States
  Ryuji Kawano, Nao Horike, Yuh Hijikata, Mio Kondo, Arnau Carné-Sánchez, Patrick Larpent, Shuya Ikemura, Toshihisa Osaki, Koki Kamiya, Susumu Kitagawa, Shoji Takeuchi and Shuhei Furukawa
  Chem, 2017, 2, 393-403, DOI: http://dx.doi.org/10.1016/j.chempr.2017.02.002 [Link] [pdf]
  
  
• Amplification and quantification of an antisense oligonucleotide from target microRNA using programmable DNA and a biological nanopore
  Moe Hiratani, Masayuki Ohara and Ryuji Kawano
  Analytical Chemistry, 2017, 89(4), 2312-2317, DOI: 10.1021/acs.analchem.6b03830 [Link] [pdf][Press]
  
  
• Expression and characterization of the Plasmodium translocon of exported proteins component EXP2
  Kazuaki Hakamada, Hirokazu Watanabe, Ryuji Kawano, Keiichi Noguchi and Masafumi Yohda
  Biochemical and Biophysical Research Communications (BBRC), 2017, 482(4), 700-705, DOI:10.1016/j.bbrc.2016.11.097 [Link]
  
  

2016

• Rhodium–Organic Cuboctahedra as Porous Solids with Strong Binding Sites
  Shuhei Furukawa, Nao Horike, Mio Kondo, Yuh Hijikata, Arnau Carné-Sánchez, Patrick Larpent, Nicolas Louvain, Stéphane Diring, Hiroshi Sato, Ryotaro Matsuda, Ryuji Kawano and Susumu Kitagawa
  Inorganic Chemistry, 2016, 55 (21), 10843–10846, DOI: 10.1021/acs.inorgchem.6b02091 [Link]
  
  
• Scalable fabrication of microneedle arrays via spatially controlled UV exposure
  ?Hidetoshi Takahashi, ?Yun Jung Heo, Nobuchika Arakawa, Tesuo Kan, Kiyoshi Matsumoto, Ryuji Kawano and Isao Shimoyama (?equal contribution)
  Microsystems & Nanoengineering, 2016, 2, 16049, DOI:10.1038/micronano.2016.49 [Link]
  
  
• Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes
  Koki Kamiya, Ryuji Kawano, Toshihisa Osaki, Kazunari Akiyoshi and Shoji Takeuchi
  Nature Chemistry, 2016, 8, 881–889, DOI:10.1038/nchem.2537 [Link]
  
  
• Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array
  Masayuki Ohara, Yusuke Sekiya and Ryuji Kawano
  Electrochemistry, 2016, 84(5), 338-341, DOI: 10.5796/electrochemistry.84.338 [Link][pdf]
  
  
• Logic Gate Operation by DNA Translocation through Biological Nanopores
  ?Hiroki Yasuga,? Ryuji Kawano, Masahiro Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki and Shoji Takeuchi
  (?equal contribution)
  PLoS One., 2016, 11(2):e0149667, DOI: 10.1371/journal.pone.0149667 [Link]
  
  
• Channel Current Analysis for Pore-forming Properties of an Antimicrobial Peptide, Magainin 1, using the Droplet Contact Method
  Hirokazu Watanabe and Ryuji Kawano
  Analytical Sciences, 2016, 32(1), 57-60, DOI: 10.2116/analsci.32.57 [Link][pdf]

2015

• Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins
  Y. Abe, K. Kamiya, T. Osaki, H. Sasaki, R. Kawano, N. Miki and S. Takeuchi
  Analyst, 2015, 140, 5557-5562, DOI: 10.1039/C4AN02201G [link]
  
  
• Towards combinatorial mixing devices without any pumps by open-capillary channels: fundamentals and applications
  M. Tani, R. Kawano, K. Kamiya & K. Okumura
  Scientific Reports, 2015, 5, 10263, DOI: 10.1038/srep10263 [link]
  
  
• Preparation of Molecule-Responsive Microsized Hydrogels via Photopolymerization for Smart Microchannel Microvalves
  Y. Shiraki, K. Tsuruta, J. Morimoto, C. Ohba, A. Kawamura, R. Yoshida, R. Kawano, T. Uragami and T. Miyata
  Macromol. Rapid Commun., 2015, 36: 515–519, DOI: 10.1002/marc.201400676 [link]
  
  
• Preparation of Structurally Colored, Monodisperse Spherical Assemblies Composed of Black and White ColloidalParticles using a Micro Flow-Focusing Device
  M. Teshima, T. Seki, R. Kawano, S. Takeuchi, S. Yoshioka and Y. Takeoka
  Journal of Materials Chemistry C, 2015, 3, 769 - 777, DOI: 10.1039/C4TC01929F [link]

2014

• A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
  R. Kawano, Y. Tsuji, K. Kamiya, T. Kodama, T. Osaki, N. Miki, S. Takeuchi
  PLoS ONE, 2014, 9(7): e102427, DOI: 10.1371/journal.pone.0102427 [Link]
  
  
• Lipid bilayers on a picoliter microdroplet array for rapid fluorescence detection of membrane transport
  T. Tonooka, K. Sato, T. Osaki, R. Kawano, S. Takeuchi
  Small, 2014, 10, No. 16, 3275–3282, DOI: 10.1002/smll.201470093 [Link] (Selected as the cover of issue)

2013

• Droplet Split-and-Contact Method for High-Throughput Transmembrane Electrical Recording
  ?Y. Tsuji,?R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
  (?equal contribution)
  Anal. Chem., 2013, 85, 10913-10919. [Link]
  
  
• Automated Parallel Recordings of Topologically Identified Single Ion Channels
  R. Kawano, Y. Tsuji, K. Sato, T. Osaki, K. Kamiya, M. Hirano, T. Ide, N. Miki, S. Takeuchi
  Scientific Reports, 2013, 3: 1995, DOI: 10.1038/srep01995. [Link]
  
  
• Round-Tip Dielectrophoresis-Based Tweezers for Single Micro-Object Manipulation
  T. Kodama, T. Osaki, R. Kawano, K. Kamiya, N. Miki, S. Takeuchi
  Biosensors and Bioelectronics, 2013, 47, 206-212. [Link]
  
  
• Droplet based lipid bilayer system integrated with microfluidic channels for solution exchange
  ?Y. Tsuji,?R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
  (?equal contribution)
  Lab on a Chip, 2013, 13, 1476-1481. [Link] (Selected as the inside cover of the issue)

2012

• A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing
  Y. Oyama, T. Osaki, K. Kamiya, R. Kawano, T. Honjoh, H. Shibata, T. Ide, S. Takeuchi
  Lab on a Chip 2012, 12, 5155-5159. (Selected as the inside cover of the issue)
  
  
• Microfluidic Control of the Internal Morphology in Nanofiber-based Macroscopic Cables
  D. Kiriya, R. Kawano, H. Onoe, S. Takeuchi
  Angewandte Chemie Int. Ed., 2012, 51, 7942-7947. (Selected as the inside cover of the issue)
  
  
• Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels
  H. Sasaki, R. Kawano, T. Osaki, K. Kamiya, S. Takeuchi
  Lab on a Chip 2012, 12, 702-704.

2011

• Simple and Stable Lipid Bilayer Formation: A Droplets Contacting Method using Parylene Micropores for Multiple Ion Channel Recordings (in Japanese)
  Y. Tsuji, R. Kawano, T. Osaki, H. Sasaki, N. Miki, S. Takeuchi
  IEEJ Trans.E. 2011, 131, 419-424.
  
  
• Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip
  R. Kawano, T. Osaki, H. Sasaki, M. Takinoue, S. Yoshizawa, S. Takeuchi
  J. Am. Chem. Soc., 2011, 133, 8474-8477.
  
  
• Electrical Access to Lipid Bilayer Membrane Microchambers for Transmembrane Analysis
  T. Osaki, Y. Watanabe, R. Kawano, H. Sasaki, S. Takeuchi
  Journal of Microelectromechanical Systems, 2011, 20, 797-799.

2010

• Lipid-coated Microdroplet Array for in vitro Protein Synthesis
  T. Osaki, S. Yoshizawa, R. Kawano, H. Sasaki, S. Takeuchi
  Anal. Chem. 2010, 83, 3186-3191.
  
  
• Quartz Nanopore Membranes for Suspended Bilayer Ion Channel Recordings
  A. Schibel, T. Edwards, R. Kawano, W. Lan, and H. White
  Anal. Chem. 2010, 82, 7259–7266.
  
  
• Parylene-coating in PDMS channels to prevent the absorption of fluorescent dyes
  H. Sasaki, H. Onoe, T. Osaki, R. Kawano, S. Takeuchi
  Sensors & Actuators: B. Chemical 2010 150, 478-482.
  
  
• A rupture detection algorithm for the DNA translocation detection through biological nanopore
  T. Osaki, J. P. Barbot, R. Kawano, H. Sasaki, O. Francais, B. Le Pioufle, S. Takeuchi
  Procedia Engineering 2010 5, 796-799.
  
  
• A Polymer-based Nanopore-integrated Microfluidic Device for Generating Stable Bilayer Lipid Membranes
  R. Kawano, T. Osaki, H. Sasaki, S. Takeuchi
  Small 2010, 6, 2100-2104. (selected as the inside cover of the issue)
  
  
• Monitoring the Escape of DNA from a Nanopore Using an Alternating Current Signal
  D. K. Lathrop, E. N. Ervin, G. A. Barrall, M. G. Keehan, R. Kawano, M. A. Krupka, H. S. White, and A. H. Hibbs
  J. Am. Chem. Soc. 2010, 132, 1878-1885.
  
  
• Solid-State Dye-Sensitized Solar Cells using Polymerized Ionic Liquid Electrolyte and Platinum-Free Counter Electrode
  R. Kawano*, T. Katakabe, H. Shimosawa, M. K. Nazeeruddinn, M. Gratzel, M. Watanabe
  Phys. Chem. Chem. Phys., 2010, 12, 1916-1921.

2009

• Controlling the Translocation of Single-Stranded DNA through α-Hemolysin Ion Channels Using Viscosity
  R. Kawano*, A. Schibel, C. Cauley, H. S. White
  Langmuir, 2009, 25 (2), 1233–1237.
  
  

2008

• Simultaneous Alternating and Direct Current Readout of Protein Ion Channel Blocking Events using Glass Nanopore Membranes
  E. N. Ervin, R. Kawano, R. J. White, H. S. White
  Anal. Chem. 2008, 80, 2069-2076.
  
  

2007

• Acceleration of　Redox Diffusion and Charge Transfer Rates in an Ionic Liquid with Nanoparticles Addition
  T. Katakabe, R. Kawano, M. Watanabe
  Electrochem. Solid-State Lett., 2007, 10, F23-F25.
  
  
• Dye-Sensitized TiO2 Solar Cells using Imidazolium-Type Ionic Liquid Crystal systems as Effective Electrolytes
  N. Yamanaka, R. Kawano, W. Kubo, N. Masaki, T. Kitamura, Y. Wada, M. Watanabe, S. Yanagida
  J. Phys. Chem. B 2007, 111, 4763-4769　(Most-Accessed Article: 9th, 2007).
  
  
• Amphiphilic ruthenium dye as an ideal sensitizer in conversion of light to electricity using ionic liquid crystal electrolyte
  R. Kawano*, Md.K. Nazeeruddin, A. Sato, M. Gr?tzel, M. Watanabe
  Electrochem. Commun. 2007, 9, 1134-1138.

2006

• Specific Charge Transport in Ionic Liquids and Ion Gels and the Importance in Material Science
  R. Kawano, H. Tokuda, T. Katakabe, H. Nakamoto, H. Kokubo, S. Imabayashi, M. Watanabe
  Jpn. J. Polym. Sci. Technol., 2006, 63, 31-40. (Review, in Japanese).
  
  

2005

• Anomaly of Charge Transport of an Iodide/Tri-iodide Redox Couple in an Ionic Liquid and Its Importance in Dye-sensitized Solar Cells
  R. Kawano, M. Watanabe
  Chem. Commun. 2005, 2107-2109.
  
  
•  Ionic Liquid Crystal as a Hole Transport Layer of Dye-Sensitized Solar Cells
  N.Yamanaka, R. Kawano, W. Kubo, T. Kitamura, Y. Wada, M. Watanabe, S. Yanagida
  Chem. Commun., 2005 , 740-742. (Selected as a Hot Article)

2004

• Dye-sensitized Solar Cells Using Ionic Liquid-based Electrolytes
  H. Matsui, K. Okada , N. Tanabe, R. Kawano and M. Watanabe
  Trans. Mater. Res. Soc. Jpn., 2004, 29, 1017-1020.
  
  
• Application of an Ionic Liquid-Based Electrolyte to a 100x100 mm2 Sized Dye-Sensitized Solar Cell
  H. Matsui, K. Okada, T. Kawashima, T. Ezure, N. Tanabe, R. Kawano and M. Watanabe
  J. Photochem. Photobio. A, Chem., 2004, 164, 129-135.
  
  
• High Performance Dye-Sensitized Solar Cells using Ionic Liquids as Their Electrolytes
  R. Kawano, H. Matsui, C. Matsuyama, A. Sato, M. A. B. H. Susan, N. Tanabe, M. Watanabe
  J. Photochem. Photobio. A, Chem., 2004, 164, 87-92.

2003

• Equilibrium Potentials and Charge Transport of an I-/I3- Redox Couple in an Ionic Liquid
  R. Kawano, M. Watanabe
  Chem. Commun. 2003, 330-331.
  
  

2000

• Intramolecular Magnetic Interaction of Phenylene-Linked Bis-β-diketone Metal Complexes
  M. M. Matsushita, T. Yasuda, R. Kawano, T. Kawai, T. Iyoda
  Chemistry Letters, 2000, No.7, 812-813.